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Comparison of Protein Fractions in Cyst Fluids and Sera

W. JAMES GARDNER, M.D.; JOHN S. COLLIS, JR., M.D.; LENA A. LEWIS, Ph.D.

Arch Neurol. 1963;8(3):291-298.

	Since this article does not have an abstract, we have provided the first 150 words of the full text PDF and any section headings.

Increased capillary permeability in tumors within the brain causes a breakdown of the blood-brain barrier¹: and in tumors within the subarachnoid space, a breakdown of the blood-cerebrospinal fluid barrier.² It seemed likely, therefore, that this capillary leakage could explain the accumulation of fluid in a cystic brain tumor. To investigate this possibility, protein fractionation studies were made on the fluids and sera of patients with cystic brain tumors.

Materials and Methods

Twenty-four patients, 12 males and 12 females, ranging in age from 7 to 67 years, who had cystic brain tumors provided the materials for study. Twenty-one had primary intracranial tumors. Three had metastatic tumors. Cyst fluid and blood were collected simultaneously at the time of operation. Total protein was determined by the biuret method³ or by the micro-Kjeldahl technique.⁴ Electrophoresis was carried out by Longsworth's⁵ modification of the Tiselius technique, using barbiturate buffer pH . . . [Full Text PDF of this Article]

Author Affiliations
CLEVELAND

From the Department of Neurological Surgery and Research Division, The Cleveland Clinic Foundation.

Footnotes
Submitted for publication June 11, 1962.

This figure is so out of line with others that it suggests possible dilution with ventricular fluid.

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