This Article  • Full text PDF  • Reply to article  • Send to a friend  • Save in My Folder  • Save to citation manager  • Permissions  Citing Articles  • Contact me when this article is cited  Related Content  • Similar articles in this journal  Social Bookmarking   What's this?

edited by James H. Eberwine, Karen L. Valentino, and Jack D. Barchas, 199 pp, with illus, $39.95, New York, NY, Oxford University Press, 1994.

Kathleen A. Maguire-Zeiss, PhD, Reviewer
Rochester, NY

Arch Neurol. 1995;52(9):854.

	Since this article does not have an abstract, we have provided the first 150 words of the full text PDF and any section headings.

This book is an excellent companion to the first book published by these editors, In Situ Hybridization: Applications to Neurobiology. The current text describes the recent methodologic challenges facing researchers who use in situ hybridization (ISH). After a brief description of the technique, subsequent chapters describe advances in more complicated applications of ISH, such as combined ISH and receptor autoradiography, dual labeling of messenger RNAs (mRNAs) and/or proteins using both isotopic and nonisotopic methods, and simultaneous analysis of peptide secretion and gene expression. This is followed by two chapters that deal with the important and timely issue of quantification of mRNAs using ISH. In addition, there are chapters that discuss the measurement of nuclear mRNAs (heteronuclear RNA) and the use of ISH in identifying alternatively transcribed mRNAs in Alzheimer's disease. In an attempt to address the issue of sensitivity when using ISH, there is a chapter devoted to the use . . . [Full Text PDF of this Article]

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter     What's this?