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Frederick J. Samaha, MD; John Gergely, MD, PhD

Arch Neurol. 1969;21(2):200-207.

	Since this article does not have an abstract, we have provided the first 150 words of the full text PDF and any section headings.

RECENT investigations have shown that actomyosin can be isolated from human muscle biopsy samples; it has a magnesium (Mg⁺⁺) moderated adenosine triphosphatase (ATPase) activity and at low ionic strength it undergoes superprecipitation, an in vitro analogue of contraction, in the presence of adenosine triphosphate (ATP), magnesium chloride and calcium chloride.¹ Both ATPase activity and superprecipitation are inhibited by removal of calcium with either a chelating agent or human fragmented sarcoplasmic reticulum. Fragmented sarcoplasmic reticulum can also be obtained from human biopsy samples² and, as in the case of rabbit muscle,^3-6 it manifests an ATP dependent calcium uptake by which it can lower the concentration of ionized calcium below the 10-⁶M threshold required for actomyosin superprecipitation.⁴ In extension of the above studies, the present work on purified myosin from normal and myotonic dystrophic human muscle was undertaken.

The procedure described is geared to the . . . [Full Text PDF of this Article]

Author Affiliations
Boston

From the Kennedy Memorial Laboratory, Massachusetts General Hospital (Dr. Samaha); the departments of neurology (Dr. Samaha) and biological chemistry (Dr. Gergely), Harvard Medical School; and the Department of Muscle Research, Retina Foundation, Institute of Biological and Medical Sciences (Dr. Gergely), Boston.

Footnotes
Submitted for publication Jan 29, 1969; accepted March 22.

Presented in part at the meeting of the American Neurological Association, Washington, DC, June 1968.

Reprint requests to the Department of Muscle Research, Retina Foundation, Institute of Biological and Medical Sciences, 20 Staniford St, Boston 02114 (Dr. Samaha).

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