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Study in Normal and Ouabain Perfused Cats

ROBERT KATZMAN, MD; LEONARD GRAZIANI, MD; RALPH KAPLAN, MD; ANTHONY ESCRIVA

Arch Neurol. 1965;13(5):513-524.

	Since this article does not have an abstract, we have provided the first 150 words of the full text PDF and any section headings.

INITIAL STUDIES in unanesthetized rhesus suggested a rapid exchange of potassium between brain and CSF.^1,2 In order to explore this relationship quantitatively, the exchange of potassium between blood, CSF, and brain has been measured in anesthetized cats, utilizing a technique of continuous perfusion of the animals' ventriculocisternal system with synthetic CSF.^3-7 By adding potassium chloride K 42 (⁴²K) to either the perfusate or blood it is possible to calculate the flux of⁴²K into or out of the CSF.^3,4 Since brain potassium exchanges very slowly with plasma K,⁵ it is possible to determine the flux into CSF of potassium from brain and blood separately. These measurements provide information regarding the mechanisms present in mammalian species including man which maintain CSF potassium within the range of 2.6 to 3.2 mM despite variations in serum potassium.^6,8,9 In the present experiments the exchange of CSF . . . [Full Text PDF of this Article]

Author Affiliations
NEW YORK

From the Saul R. Korey Department of Neurology, Albert Einstein College of Medicine, Bronx, NY.

Footnotes
Submitted for publication July 15, 1965; accepted July 27.

Reprint requests to Department of Neurology, Albert Einstein College of Medicine, New York, NY 10461 (Dr. Katzman).

The symbols used in this paper are as follows: Vi ml/ minute—inflow rate of ventricular perfusate; Vo ml/minute—outflow rate of cisternal effluent; Vf ml/minute—formation rate of new CSF by choroid plexus; Va ml/minute —absorption rate of CSF by arachnoid villi, etc; Kf mEq/ liter—potassium concentration of freshly formed CSF; Ki mEq/liter—influx of potassium concentration (ventricular perfusate); Ko mEq/liter—outflow of potassium concentration (cisternal effluent); KpI mEq/liter—potassium concentration of plasma; Ci, Co mg/100 cc—concentration of ouabain in perfusate and effluent; kvp+vBr ml/ minute—transport coefficient of 42K, ventricle to plasma plus brain; expressed as ml of CSF cleared of 42K/minute. This coefficient has also been referred to by others as kDX5 and kvi12; kpv ml/minute—transport coefficient, 42K, plasma to ventricle, expressed as milliliters of plasma cleared of 42K/ minute; Mpv mmoles/minute-—unidirectional flux of potassium, plasma to CSF; MBrv µM/minute—unidirectional flux of potassium, brain to CSF.

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