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Glucocerebrosidase Mutations and Risk of Parkinson Disease in Chinese Patients
Eng-King Tan, MD;
Justina Tong, BSc;
Stephanie Fook-Chong, MSc;
Yuen Yih, MD;
Meng-Cheong Wong, MD;
Ratnagopal Pavanni, MD;
Yi Zhao, MD
Arch Neurol. 2007;64(7):1056-1058.
Gaucher disease (GD) is a recessive inherited glycolipid storage disorder caused by a deficiency of the lysosomal enzyme glucocerebrosidase (GBA).1 Recent observation of an association between Parkinson disease (PD) and GBA gene mutations in Ashkenazi Jewish patients has generated considerable scientific interest.2 However, data on this association in non–Ashkenazi Jewish populations is still limited.3-4 Two recent studies in white patients with PD reported a negative or questionable association with GBA mutations.3-4 The GBA L444P mutation appears to be the most common mutation among Chinese patients with GD in Taiwan.5 However, GD is extremely rare in our country. To our knowledge, there has not been a single reported case of GD in our population. Using a case-control methodology, we conducted a genetic screen of 2 common GBA mutations (L444P and N370S) in our ethnic Chinese patients with PD.
Methods
Our study included consecutive ethnic Chinese patients diagnosed with idiopathic PD by movement disorders neurologists at a tertiary referral center in Singapore. The PD diagnosis was made in accordance with the UK Parkinson's Disease Society Brain Bank clinical criteria. Controls were healthy individuals of similar age, sex, and race without neurodegenerative diseases and were examined by us. The institutional ethics committee approved the study, and informed consent was obtained from all study subjects. We extracted DNA from lymphocytes according to a standardized protocol. Allelic discrimination using the 5' nuclease activity assay was adapted to detect the L444P and N370S mutations. Primers and probes were designed using Primer Express 1.5 software (Applied Biosystems, Foster City, California). The amplification reactions were carried out in an ABI Prism 7700 Sequence Detection System (Applied Biosystems) with 2 initial steps (50°C for 2 minutes followed by 95°C for 10 minutes) and 40 cycles of a 2-step polymerase chain reaction (95°C for 15 seconds, 60°C for 1 minute). After the polymerase chain reaction, allelic-specific fluorescence was measured on the ABI Prism 7700 Sequence Detection System (Applied Biosystems) using Sequence Detection System 1.7 software for allelic discrimination. Sequence analysis was used to confirm the genotypes for all the positive samples (Figure). The numerical and categorical variables were compared using a Fischer exact test and t test. Statistical significance was defined at P<.05.
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Figure. Sequence chromatograph showing the T-to-C mutation (L444P) (position indicated by vertical line).
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Results
A total of 678 subjects comprising 331 patients with PD and 347 controls similar in age and sex were analyzed. The mean ± SD age was 70.0 ± 11.0 years (range, 39-98 years) and 51.4% were men. The mean ± SD ages at onset of patients with PD and controls was 64.0 ± 10.0 years (range, 35-95 years) and 64.0 ± 12.0 years (range, 29-90 years), comprising 48.7% men.
The frequency of the L444P and N370S mutations in patients with PD and controls was 8 of 331 vs 0 of 347 (2.4% vs 0.0%, P = .003). All 8 patients with PD were heterozygous for the L444P mutation. However, the N370S mutation was not detected in all 778 subjects. Subset analysis revealed the association to be stronger in the group of subjects younger than 55 years (12.1% vs 0.0%, P = .004) compared with those older than 55 years (1.3% vs 0.0%, P = .06). The age at onset was lower in patients with PD with the L444P mutation compared with those without (49.0 ± 13.0 years; range, 29-70 years; vs 64.0 ± 11.0 years; range, 31-90 years; P = .002), and more women were present in the former group (87.5% vs 47.7%, P = .03).
Almost all patients with the L444P mutation presented with typical features of PD that were responsive to dopaminergic agents, and some developed levodopa-induced dyskinesias (Table). Previous mutational screening of the PARK1, PARK2, PARK6, PARK7, and PARK8 genes in these patients showed unremarkable findings.
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Table. Clinical Features of Patients With Parkinson Disease (PD) With GBA Mutations
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Comment
We found a significantly higher prevalence of the L444P mutation in our patients with PD compared with controls, and the association was stronger in younger subjects and in women. The zero prevalence in the controls appeared compatible with the extreme rarity of GD in our population. Six of the 8 affected patients with PD did not have a family history of PD and the remaining 2 were siblings. The mean age at onset of these patients was significantly younger than the age of patients with PD without GBA mutations. One study in Ashkenazi Jewish patients reported a younger age at onset in PD with mutations, but this was not observed by others.2, 6
A wide spectrum of neurological manifestations, such as epilepsy, cerebellar ataxia, psychiatric symptoms, dementia, and parkinsonism, has been reported in GD. GBA mutations have also been reported in other Lewy body disorders.7 The typical PD presentation and disease progression in all our 8 affected patients with PD was compatible with recent observations in Ashkenazi Jewish patients. The pathophysiologic link between PD and GD could be due to the impaired degradation of  -synuclein aggregation as a result of lysosomal and proteasomal dysfunction.
Although the 2.4% frequency of the L444P GBA mutation in our patients with PD may appear low, this finding is significant due to the extreme rarity of GD in our population, supported by confirmation of the absence of this mutation among our healthy controls. While we cannot exclude the contribution of other rare GBA mutations, our study suggests that the L444P GBA mutation is associated with an increased risk of PD among the Chinese population.
AUTHOR INFORMATION
Correspondence: Dr Tan, Department of Neurology, Singapore General Hospital, Outram Road, Singapore 169608 (gnrtek{at}sgh.com.sg).
Author Contributions: Acquisition of data: Tan, Tong, Fook-Chong, Yih, Wong, Pavanni, and Zhao. Analysis and interpretation of data: Tan. Drafting of the manuscript: Tan. Critical revision of the manuscript for important intellectual content: Tan, Tong, Fook-Chong, Yih, Wong, Pavanni, and Zhao. Administrative, technical, and material support: Tan, Tong, Fook-Chong, Yih, Wong, Pavanni, and Zhao. Study supervision: Tan, Tong, Fook-Chong, Yih, Wong, Pavanni, and Zhao.
Financial Disclosure: None reported.
Funding/Support: This study was supported by the National Medical Research Council and SingHealth.
Additional Contributions: Lee Wei-Ling, MD, and Ivy Ng, MD, provided assistance and feedback.
REFERENCES
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