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A Quantitative Study of Water Diffusion in Multiple Sclerosis Lesions and Normal-Appearing White Matter Using Echo-Planar Imaging
Massimo Filippi, MD;
Giuseppe Iannucci, MD;
Mara Cercignani, PhD;
Maria Assunta Rocca, MD;
Arianna Pratesi;
Giancarlo Comi, MD
Arch Neurol. 2000;57:1017-1021.
ABSTRACT
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Objectives We used an optimized echo-planar pulse sequence for isotropically weighted diffusion imaging (1) to measure mean diffusivity ( ) in lesions and normal-appearing white matter (NAWM) in the entire brain from patients with mildly disabling relapsing-remitting multiple sclerosis (MS), (2) to compare the of NAWM from patients with that of white matter from normal controls, (3) to evaluate whether lesions classified on the basis of their appearance on enhanced T1-weighted scans have different , and (4) to investigate the relationship between diffusion changes in lesions and NAWM.
Methods Dual-echo and diffusion-weighted scans were obtained from 35 patients with relapsing-remitting MS and 24 sex- and age-matched normal controls. Postcontrast T1-weighted images were also obtained from the patients. After creating maps and image coregistration, values were measured for MS lesions larger than 5 mm and for 22 NAWM areas from each subject.
Results All NAWM areas studied had significantly higher in patients than in controls. A total of 173 lesions were identified on the dual-echo scans from patients. The average for these lesions was significantly higher than that of NAWM. Twenty-two lesions were enhancing and 60 were classified as T1-hypointense. No significant difference in values was found between enhancing and nonenhancing lesions, while the average of T1-hypointense lesions was significantly higher than the average of T1-isointense lesions. There was no significant correlation between the average in lesions and NAWM.
Conclusions This study shows that diffusion-weighted imaging is able to identify MS lesions with severe tissue disruption. It also shows that widespread increased diffusion can be measured in the NAWM from patients with MS, and suggests that such changes are, at least partially, independent of larger abnormalities.
INTRODUCTION
IN MULTIPLE SCLEROSIS (MS), conventional magnetic resonance imaging (MRI) has proved to be sensitive in detecting lesions and their changes over time.1-2 However, conventional MRI is not without relevant limitations. These include the lack of specificity to the heterogeneous pathologic substrates of MS lesions3-5 and the inability to detect subtle abnormalities in the so-called normal-appearing white matter (NAWM).5
Diffusion-weighted imaging (DWI) is a promising tool for overcoming the limitations of conventional MRI. It provides a unique form of magnetic resonance contrast that enables diffusion of water molecules to be measured and, as a consequence, provides information about the orientation, size, and geometry of brain structures.6 If water molecules are free to move in any direction from a certain position, they will spread out over time, and the magnitude of the distance moved is characterized by the molecular self diffusion coefficient. Measurements of water diffusion in vivo can be easily performed by MRI in terms of an apparent diffusion coefficient (ADC), which incorporates the effects of all the intravoxel incoherent motions, including molecular diffusion and microcirculation in capillaries.7 Cellular structures in the central nervous system restrict water molecular motion, and therefore the distance moved over time seems reduced. Hence, the ADC measured in vivo is always lower than the true diffusion coefficient of water at body temperature.8 Pathologic processes that modify tissue integrity, thus reducing "restricting" barriers, can result in increased ADC. Because some cellular structures such as axons are aligned on the scale of an image pixel, the restriction and ADC are also dependent on the direction in which diffusion is measured, thus making comparison of ADC values meaningless without taking into account the measurement direction. One measurement of diffusion that is independent of the orientation of structures is provided by measuring the ADC in 3 orthogonal directions, and then averaging the results to form the mean diffusivity ().
Preliminary studies showed that it is possible to quantify the tissue damage in MS lesions visible on T2-weighted scans, but these studies were limited by the small and heterogeneous samples studied and partial brain coverage.9-13 Many of them9-12 did not use echo-planar imaging (EPI) technology and DWIs were frequently degraded by motion artifacts. On the contrary, EPI-DWIs are prone to geometric distortions due to eddy currents induced by the strong gradient pulses applied. We recently developed an EPI pulse sequence for isotropically weighted diffusion imaging optimized to give maximum signal-to-noise ratio in the computed trace image and designed to produce inherently low distortions.14 In this study, we used this sequence to measure in lesions and NAWM in the entire brain from a relatively large sample of patients with mildly disabling relapsing-remitting MS. Aims of this study were (1) to compare the of the NAWM from patients with that of the white matter from age- and sex-matched normal controls, (2) to evaluate whether lesions classified on the basis of their appearance on enhanced T1-weighted scans have different , and (3) to investigate the relationship between diffusion changes in NAWM and lesion extent and severity to elucidate the nature of NAWM changes in MS.
PATIENTS AND METHODS
SUBJECTS
We studied 35 patients with clinically definite, relapsing-remitting MS15-16 (20 women and 15 men). Their mean (SD) age was 28.0 (4.8) years; median duration of the disease, 3.5 years (range, 1-8 years); and median Expanded Disability Status Scale score,17 1.5 (range, 1.0-3.0). To be included in the study, all patients had to have been untreated with immunosoppressive or immunomodulatory drugs for at least 1 year before study entry. They also should not have had relapses and steroid treatment in the 3 months preceding the study. Twenty-four healthy volunteers (15 women and 9 men; mean [SD] age, 29.0 [3.7] years) served as controls. Local ethical committee approval and written informed consent from all the subjects were obtained before study initiation.
MAGENETIC RESONANCE IMAGING
Brain MRI scans were obtained from patients and controls using a scanner operating at 1.5 T. During a single session, the following scans were performed without moving the subject from the scanner: (1) dual-echo conventional spin echo (repetition time [TR], 3300 milliseconds; first echo echo time [TE], 30 milliseconds; second echo TE, 80 milliseconds; number of acquisitions, 1); (2) spin echo echo-planar (interecho spacing, 0.8; TE, 160 milliseconds), collecting 1 T2-weighted image and 3 DWIs for each slice, with identical diffusion-encoding waveforms, designed to give an attenuation dependent only on the trace of the diffusion tensor (Tr{D}) and a resulting b factor of 289 s/mm2, in each of the read, phase-encoded, and slice-selection directions (isotropically weighted imaging); and (3) T1-weighted conventional spin echo (TR, 680 milliseconds; TE, 17 milliseconds; number of acquisitions, 2) 10 minutes after the injection of 0.1-mmol/kg gadolinium-DTPA (this sequence was not obtained from controls).
For the dual-echo and the T1-weighted scans, 24 contiguous interleaved axial slices were acquired with a 5-mm slice thickness, a 256x256 matrix, and a 250x250-mm field of view, giving an in-plane spatial resolution of about 1x1 mm. The set of slices for the echo-planar DWIs was the same used for the dual-echo, with a 128 x 128 matrix and a 250x250-mm field of view. The manufacturer's own phase correction and regridding algorithm were used before Fourier transformation and interpolation to a 256x256 image matrix.
IMAGE ANALYSIS AND POSTPROCESSING
Lesions were identified on the proton density–weighted scans and marked by agreement on the hard copies by 2 observers who were unaware of the patients' clinical status. T2-weighted images were always used to increase confidence in lesion identification. To minimize partial volume effects, only lesions with a diameter greater than 5 mm underwent subsequent analysis. Using postcontrast T1-weighted scans, we classified these lesions as enhancing or nonenhancing, according to published criteria.18 Nonenhancing lesions were classified as T1 isointense or hypointense. T1 hypointensity was defined as reduced lesion signal intensity with respect to surrounding NAWM. A single observer, unaware of lesion classification, displayed the dual-echo images on a computer screen and, using marked hard copies as a reference, outlined these lesions on the proton density–weighted images and measured lesion volumes using a semi-automated segmentation technique based on local thresholding.19 The outlined regions of interest were then mapped onto the coregistered maps, and the areas and of each lesion measured. Coregistration of images was performed using a surface-matching technique, based on mutual information.20 Mean diffusivity maps were obtained as follows: The average of the 3 DWIs was calculated to improve the signal-to-noise ratio. Images were then realigned to correct for misregistration due to eddy currents. Finally, an image of the (Tr{D}/3) was produced by performing the following calculation on a pixel-by-pixel basis:
=-(1/3b)ln (Sav/S1)
where Sav is the average DW signal intensity and S1 is the T2-weighted signal intensity (with b  0) (Figure 1). The volume of T1-hypointense lesions was measured with the same segmentation technique used for measuring lesion volumes on proton density–weighted images.19
Mean diffusivity values of NAWM in different brain regions were also studied; the NAWM areas were selected on the dual-echo scans. They did not have to have adjacent lesions either in the same slice or in the slices above and below. Whenever possible, square regions of interest of uniform size (3x3 pixels) were placed bilaterally in the white matter of the following areas: the pons, the cerebellar hemispheres, the internal capsules, the anterior and posterior portions of the corpus callosum, and the temporal, parietal, occipital, and frontal lobes (in regions far from ventricles and cortical gray matter). Two regions of interest were also placed close to the anterior and posterior parts of the body of the lateral ventricles, and one close to the cortical gray matter of the frontal lobe. The outlined NAWM regions were then transferred onto the coregistered maps. Using the same method, the values of the same brain regions were measured from controls.
STATISTICAL ANALYSIS
A 2-tailed t test for not-paired data was used to compare (1) the of the NAWM from patients with that of the white matter from controls and (2) the of the NAWM with that of lesions from patients. The Mann-Whitney test was used to compare (1) the and area of enhancing vs nonenhancing lesions and (2) the and area of T1-hypointense vs T1-isointense lesions. Univariate correlations were assessed using the Spearman rank correlation coefficient.
RESULTS
Diffusion-weighted images were of good quality for all patients and controls and were not affected by motion artifacts or geometric distortions. The average of the 747 NAWM areas from patients was 0.821x10-3 mm2/s (SD, 0.18x10-3 mm2/s) and was significantly higher (P<.001) than that of the 528 white matter areas from controls (0.727 x 10-3 mm2/s [SD, 0.09x10-3 mm2/s]). In Table 1, the average values and SDs of the NAWM for each of the anatomical regions studied from patients and controls are reported. Patients with MS had significantly higher values in all the areas studied.
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Average (SD) Mean Diffusivity () in Different White Matter Areas From Controls and Normal-Appearing White Matter Areas From Patients With Multiple Sclerosis (MS)*
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A total of 173 lesions were identified on the dual-echo scans from patients. The average for these lesions was 1.085x10-3 mm2/s (SD, 0.10 x10-3 mm2/s) and was significantly higher (P<.001) than the average of NAWM. Twenty-two lesions were enhancing, and 60 were classified as T1-hypointense. No significant difference in was found between enhancing (mean [SD], 1.039 [0.17]x10-3 mm2/s) and nonenhancing lesions (1.040 [0.14]x10-3 mm2/s), while the average of T1-hypointense lesions (1.115 [0.13]x10-3 mm2/s) was significantly higher (P<.001) than the average (0.995 [0.13]x10-3 mm2/s) of T1-isointense lesions (Figure 2). Mean lesion volumes were similar for enhancing and nonenhancing and for T1-hypointense and T1-isointense lesions. There was no significant correlation between the average values in lesions and NAWM (r=0.18). Moderate correlations were found between the average in NAWM and the T2-hyperintense and T1-hypointense lesion volumes (r=0.4; P=.03). There were no significant correlations between average values in lesions and NAWM and patients' age, disease duration, and Expanded Disability Status Scale level.17
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Figure 2. Means and percentile distributions of mean diffusivity for T1-isointense and T1-hypointense multiple sclerosis lesions. The bold bars represent the means; the upper and lower rectangles, the 75th and 25th percentiles, respectively; and the upper and lower bars, the 90th and 10th percentiles, respectively.
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COMMENT
Diffusion-weighted imaging is able to probe structural properties of tissue (including the size, shape, integrity, and orientation of water spaces) that are inaccessible to other MRI techniques. The pathologic elements of MS can alter the permeability or geometry of structural barriers to water diffusion in the brain. Therefore, although DWI has been mostly used for the early detection of cerebral ischemia,21-23 a promising application of the technique is its use to quantify the structural damage in MS. In this study, we measured values of lesions and different NAWM areas using an optimized EPI-DWI sequence to elucidate the nature of brain damage in patients with MS. We assessed patients with early relapsing-remitting MS and a very narrow range of disability, and these are likely to be the main reasons why a correlation between values and disease duration or level of disability was not found. Although this issue warrants further investigation, we believe that our results confirm the potential of DWI to elucidate in vivo the nature of the damage in MS lesions and NAWM and provide important information about the pathophysiological characteristics of MS during its early phases.
We found that the average diffusivity is higher in the NAWM from patients with mildly disabling relapsing-remitting MS than in the white matter from controls. Although this indicates a net loss of structural barriers to water molecular motion in the NAWM, we can only speculate on the possible pathologic substrates of this finding. Subtle changes are known to occur in the NAWM from patients with MS, including diffuse astrocytic hyperplasia, patchy edema, perivascular infiltration, gliosis, abnormally thin myelin, and axonal loss.24-26 Since "inflammatory" changes and gliosis could potentially restrict water molecular motion, we believe that myelin and axonal loss are the most likely contributors to the increased we observed in the NAWM. Results from postmortem,26 magnetic resonance spectroscopy,27-28 and magnetization transfer imaging (MTI)29-31 studies support the concept that tissue destruction can occur in the NAWM of patients with MS and suggest that this might contribute to patients' clinical status.27-29,31 In addition to previous observations, this study provides systematic evaluation of different brain regions and indicates that NAWM changes in MS are widespread. Interestingly, such abnormalities seem to be more severe in sites such as the corpus callosum, the internal capsules, or the periventricular and subcortical regions, where macroscopic MS lesions are also usually located.
In our sample, the of NAWM was not significantly correlated with the of macroscopic lesions and was only moderately correlated with lesion extent on T2- and T1-weighted images. This suggests that subtle NAWM changes are not merely the result of wallerian degeneration of axons transversing larger lesions, but they more likely represent small focal abnormalities beyond the resolution of conventional scanning and independent of larger lesions. This agrees with the demonstration that tiny MS lesions can be seen when using high field magnets32 or thin slices.33 If confirmed, this finding indicates that measuring in NAWM and lesions may provide partially independent and complementary data for assessing brain abnormalities from patients with MS.
Mean diffusivity values are highly variable in MS lesions and higher than those from NAWM. This indicates that variable degrees of tissue damage occur within MS lesions, and confirms the results of previous studies obtained from patients in more advanced phases of the disease.9-13 We measured the highest in T1-hypointense lesions. Previous studies showed that T1-hypointense lesions are those in which severe tissue loss has occurred,4, 34 and their extent is correlated with disease progression in patients with secondary progressive MS.35 Although postmortem studies correlating histopathologic characteristics and DWI changes are needed, the observation that T1-hypointense lesions have the highest suggests that axonal loss and demyelination are the most likely pathologic substrates of increased in MS. We did not find any difference in the between enhancing lesions and nonenhancing lesions. Previous studies achieved conflicting results on this aspect. A navigated spin-echo diffusion study12 found no difference in between enhancing and nonenhancing lesions, but other studies10, 13 found higher diffusivity in enhancing or acute lesions. However, in one study10 only 2 acute lesions were studied, while in the other,13 although water diffusion was measured from 15 enhancing lesions, they were all from a small group of patients with different clinical courses. In acute (enhancing) lesions, vasogenic edema, demyelination, and axonal loss27 can determine increased , while remyelination, presence of inflammatory cells, and myelin breakdown products36 can create "restricting" barriers. In chronic (nonenhancing) lesions, tissue loss can increase values, while gliosis or low-grade inflammatory processes37 can act as restricting barriers. This suggests that DWI gives complementary information to T1-weighted images, with the advantage that it provides quantitative measurements. Our results also support the concept that enhancing and nonenhancing lesions have highly heterogeneous pathologic substrates with different effects on water molecular motion.
In conclusion, our results show that severe structural changes can occur in MS lesions from the early phases of the disease. They also indicate that changes in water diffusivity, although milder, can be detected in several areas of NAWM and are more pronounced in those areas that are typical sites of MS macroscopic lesions. These observations call for therapeutic interventions to prevent tissue loss in MS early in the course of the disease. Longitudinal studies are now warranted to assess whether an increased proportion of lesions with markedly increased or larger portions of NAWM with abnormal are associated with an increased risk of a subsequent unfavorable evolution of the disease.
AUTHOR INFORMATION
Accepted for publication December 6, 1999.
Dr Iannucci is supported by a grant from the Neurology School, University of Chieti, Chieti, Italy.
Reprints: Massimo Filippi, MD, Neuroimaging Research Unit, Department of Neuroscience, Scientific Institute Ospedale San Raffaele, Via Olgettina, 60, 20132 Milan, Italy (e-mail: filippi.massimo{at}hsr.it).
From the Neuroimaging Research Unit (Drs Filippi, Iannucci, Cercignani, and Rocca and Ms Pratesi) and the Clinical Trials Unit (Dr Comi), Department of Neuroscience, Scientific Institute Ospedale San Raffaele, University of Milan, Milan, Italy.
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