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Complementary DNA Libraries and Neurological Disease
Benjamin Joseph, BA;
Henry Furneaux, PhD
Arch Neurol. 1998;55:785-788.
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INTRODUCTION
The DNA in our cell nuclei contains all the information necessary to direct our biological functions. The gene is 1 unit of such information that usually specifies the expression of 1 protein product. A detailed knowledge of each gene will enable us to understand disease at the molecular level. To study each gene we must purify and study it in isolation. This process is usually described as gene cloning. However, this is a difficult task since most of the human genome is junk DNA that does not encode genes. Messenger RNA (mRNA), on the other hand, is representative of only that DNA that has been transcribed. More to the point, within each tissue or cell type, only mRNA that is important for the function of the selected cells will be present. Indeed, different cell types, developmental stages, and many disease states arise because of . . . [Full Text of this Article]
CLONING A GENE USING A cDNA LIBRARY
CLONING BY DNA ANNEALING: ISOLATION OF THE SCRAPIE GENE
CLONING AN ANTIGEN: THE PARANEOPLASTIC ENCEPHALOMYELITIS ANTIGEN
INTERACTION CLONING: THE HUNTINGTIN-ASSOCIATED PROTEIN
SUMMARY AND FUTURE DEVELOPMENTS
From the Program in Molecular Pharmacology and Therapeutics, Memorial Sloan Kettering Cancer Center, New York, NY.
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