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Expression of 'Alzheimer Antigens' in Cultured Skin Fibroblasts
John P. Blass, MD, PhD;
Andrea C. Baker;
Li-wen Ko, BVM, PhD;
Rex K.-E. Sheu, PhD;
Ronald S. Black, MD
Arch Neurol. 1991;48(7):709-717.
Abstract
• When cultured skin fibroblasts were exposed to culture conditions designed to favor the expression of neuronal antigens, cells from each of 19 patients with Alzheimer's disease reacted immunocytochemically with antibodies to paired helical filaments, Alz-50, or both compared with only a small fraction of cells from 19 identically treated age-matched control cultures. Immunoblots confirmed the presence of soluble material reacting with Alz-50 antibody in the Alzheimer fibroblasts. Ultrastructurally, fascicles of 10-nm filaments were seen that occasionally twisted around each other, but no structures were seen that were identical to paired helical filaments. Thus, cultured skin fibroblasts from patients with Alzheimer's disease developed greater immunocytochemical reactivity with antibodies raised to paired helical filaments than did fibroblasts from control subjects, when cultured under the specified conditions.
Author Affiliations
From the Altschul Laboratory for Dementia Research, Cornell University Medical College at Burke Medical Research Institute, White Plains, NY.
Footnotes
Accepted for publication December 17, 1990.
Presented in part at the Dahlem Conference, Berlin, Federal Republic of Germany, December 1987; at the annual meetings of the Society for Neuroscience, New Orleans, La, November 20, 1987 and Toronto, Ontario, November 17, 1988; at the International Conference on Alzheimer's Disease, Las Vegas, Nev, September 5, 1988; at La Fondation Ipsen Conference, Toulouse, France, April 24,1989; and at the International Psychogeriatric Congress, Tokyo, Japan, September 8,1989.
Reprint requests to the Altschul Laboratory for Dementia Research, Cornell University Medical College at Burke Medical Research Institute, 785 Mamaroneck Ave, White Plains, NY 10605 (Dr Blass).
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