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Quantitation by Immunoenzyme Microassay With Panel of Monoclonal Antibodies

Sten Fredrikson, MD, PhD; Jan Ernerudh, MD, PhD; Tomas Olsson, MD, PhD; Pia Forsberg, MD, PhD; Hans Link, MD, PhD

Arch Neurol. 1989;46(4):372-375.

Abstract
• Phenotypic distribution of mononuclear cells in cerebrospinal fluid (CSF) and peripheral blood from patients with multiple sclerosis (MS) and, for reference, patients with acute aseptic meningoencephalitis (AM), and in blood only from healthy controls, was studied with an immunoenzymatic microassay enabling analysis even in the presence of a normal CSF cell count. In MS, increased CD5⁺ (pan-T) cell proportion in CSF compared with blood was not reflected by changes of CD4⁺ or CD8⁺ cells, while in AM, an increase of CD4⁺ cells was registered. Therefore, a population of CD5⁺, CD4^-, and CD8^- cells may be anticipated to exist in CSF of patients with MS. Numbers of OKB7⁺, OKM1⁺, or HLA-DR⁺ cells did not distinguish between MS and AM. Proliferating cells expressing transferrin receptors (OKT9⁺ cells) were generally few or absent in CSF and not useful as a marker of disease activity in either MS or AM.

Author Affiliations
From the Department of Neurology, Karolinska Institutet, Huddinge University Hospital, Stockholm (Drs Fredrikson, Olsson, and Link), and the Departments of Neurology (Dr Ernerudh) and Infectious Diseases (Dr Forsberg), Linköping (Sweden) University Hospital.

Footnotes
Accepted for publication Sept 15, 1988.

Reprint requests to the Department of Neurology, Karolinska Institutet, Huddinge University Hospital, S-141 86 Huddinge, Stockholm, Sweden (Dr Fredrikson).

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