 |
|
Anti-Purkinje Cell Antibody Producing B-Cell Lines From a Patient With Paraneoplastic Cerebellar Degeneration
Tetsuro Tsukamoto, MD;
Osamu Yoshie, MD;
Kohtaro Tada;
Yuzo Iwasaki, MD
Arch Neurol. 1987;44(8):833-837.
Abstract
| |
• Two patients with paraneoplastic cerebellar degeneration with anti-Purkinje cell antibodies are described. Using an avidin-biotin peroxidase complex method, serum anti-Purkinje cell antibody titers of these patients were 1:32 000 and 1:64000, and the cerebrospinal fluid titers were 1:40 and 1:320, respectively. After extensive plasmapheresis together with radiation therapy and chemotherapy, the cerebellar symptoms of one patient ameliorated concomitantly with a reduced serum anti-Purkinje cell antibody titer. Four B-cell lines producing anti-Purkinje cell antibodies were established from one patient by Epstein-Barr virus trasformation of peripheral lymphocytes. None of these antibodies reacted with peripheral T cells, although the serum anti-Purkinje cell activity of the patient was markedly reduced after absorption with purified human T cells. Thus, anti-Purkinje cell antibodies are apparently directed both to antigens unique to the cerebellar tissue and those shared by T cells.
Author Affiliations
From the Departments of Neurology (Dr Tsukamoto), Bacteriology (Dr Yoshie and Mr Tada), and Neuropathology (Dr Iwasaki), Tohoku University School of Medicine, Sendai, Japan.
Footnotes
Accepted for publication Feb 23, 1987.
Reprint requests to Department of Neurology, Institute of Brain Diseases, Tohoku University School of Medicine, Seiryo-machi 1-1, Sendai, 980, Japan (Dr Tsukamoto).
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati
What's this?
THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES
Ultrastructural Localization of Anti-Purkinje Cell Antibody--Binding Sites in Paraneoplastic Cerebellar Degeneration
Hida et al.
Arch Neurol 1994;51:555-558.
ABSTRACT
Antineural Autoantibodies in Patients With Paraneoplastic Cerebellar Degeneration
Tsukamoto et al.
Arch Neurol 1989;46:1225-1229.
ABSTRACT
|
