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Valerie Askanas, MD, PhD; Ghislaine Gallez-Hawkins, MS

Arch Neurol. 1985;42(8):749-752.

Abstract
• In two- to five-week tissue cultures of biopsied adult human skeletal muscle, combined addition to the culture medium of insulin, fibroblast growth factor, and epidermal growth factor synergistically increased creatine kinase activity 17-fold, increased acetylcholine receptors tenfold, and accelerated muscle differentiation. This study provides the first demonstration of the beneficial influence of these peptides on human muscle. It also establishes a new culture medium, resulting in the following: (1) much better longterm growth and differentiation of biopsied adult human muscle; and (2) by allowing elimination of embryo extract and reduction of serum, an important step toward developing a fully defined medium for culturing biopsied adult human normal and pathologic muscle tissue.

Author Affiliations
From the USC Neuromuscular Center, University of Southern California School of Medicine, Los Angeles.

Footnotes
Accepted for publication Oct 1, 1984.

Reprint requests to USC Neuromuscualr Center, University of Southern California School of Medicine, 637 S Lucas Ave, Los Angeles, CA 90017 (Dr Askanas).

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