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Micromethod for Detection of Oligoclonal IgG in Unconcentrated CSF by Polyacrylamide Gel Electrophoresis
Matti V. Iivanainen, MD, PhD;
William Wallen, PhD;
Marta E. Leon, PhD;
Jormak Keski-Oja, MD;
Vincent P. Calabrese, MD;
Mary A. Krasny;
Edward A. Waybright, MD;
John B. Selhorst, MD;
John W. Harbison, MD;
David L. Madden, DVM, PhD;
John L. Sever, MD, PhD
Arch Neurol. 1981;38(7):427-430.
Abstract
• A micromethod to detect oligoclonal IgG from 50 µL of unconcentrated CSF was developed by using polyacrylamide gel electrophoresis in sodium dodecyl sulfate (SDS-PAGE). Of 17 patients with multiple sclerosis, oligoclonal bands were demonstrated in 16 instances (94%) by micro-SDS-PAGE and in 13 (76%) by agarose gel electrophoresis. The corresponding figures among 30 patients with optic neuritis were 16 (54%) and five (17%), respectively, and among ten patients with other neurological disease the figures were two (20%) and none, respectively. Thus, micro-SDS-PAGE is more sensitive than agarose gel electrophoresis for detection of oligoclonal IgG. The small volume of unconcentrated CSF that is required enhances the usefulness of this test.
Author Affiliations
From the Infectious Diseases Branch (Drs Iivanainen, Wallen, Leon, Calabrese, Krasny, Madden, and Sever), and the National Cancer Institute (Dr Keski-Oja), the National Institutes of Health, Bethesda, Md; and the Department of Neurology (Drs Calabrese, Waybright, Selhorst, and Harbison), Medical College of Virginia, Richmond. Drs Iivanainen and Keski-Oja are currently with the Department of Neurology, University of Helsinki, Finland.
Footnotes
Accepted for publication Sept 17, 1980.
Presented in part at the 32nd annual meeting of the American Academy of Neurology, New Orleans, May 2, 1980.
Reprint requests to Infectious Diseases Branch, Bldg 36, Room 5D-06, National Institutes of Health, Bethesda, MD 20205 (Dr Madden).
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