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The Enzymatic-Isotopic Method vs Liquid Chromatography

Sami I. Harik, MD; Laurence J. Marton, MD

Arch Neurol. 1981;38(2):91-94.

Abstract
• Twenty-six samples of CSF were assayed for their putrescine content by three analytical techniques: total and free putrescine levels were assayed by fluorometry after separation by column chromatography and compared with putrescine levels as assayed by the enzymaticisotopic method. Putrescine levels obtained by the enzymatic-isotopic method correlated relatively well with free putrescine levels, but grossly underestimated the results obtained for total putrescine values. The difference between total and free putrescine values is mostly due to metabolites of putrescine, such as monoacetylputrescine, which account for about 40% of the total putrescine value. The importance of the free putrescine level as a marker of CNS malignant neoplasms in comparison to that of the total putrescine level deserves investigation.

Author Affiliations
From the Department of Neurology, University of Miami (Fla) School of Medicine (Dr Harik); and the Brain Tumor Research Center, Department of Neurological Surgery, and the Department of Laboratory Medicine, University of California, San Francisco (Dr Marton).

Footnotes
Accepted for publication Jan 31, 1980.

Reprint requests to Department of Neurology, University of Miami School of Medicine, PO Box 016960, D4-5, Miami, FL 33101 (Dr Harik).