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Its Use in Routine Diagnostic Muscle Biopsies

Daniel P. Perl, MD; Brian W. Little, MD, PhD

Arch Neurol. 1980;37(10):641-644.

Abstract
• Acridine orange-RNA fluorescence in diagnostic muscle biopsies is a sensitive, specific method to identify single fibers undergoing neurogenic atrophy. Fibers thus identified may be clearly distinguished from nonfluorescent type-specific atrophic fibers, as well as from atrophic fibers encountered in other myopathic conditions. Regenerating fibers, inflammatory cells, and mast cells stain prominently with acridine orange but are easily identified by their morphologic features. Acridine orange-RNA fluorescence is extremely useful in the evaluation of early neurogenic atrophy without fiber-type grouping and may be performed on paraffin-embedded sections, thus allowing retrospective studies. In addition, this technique may provide clues to the pathogenesis of neuromuscular disease that involves altered nucleic acid metabolism.

Author Affiliations
From the Pathology Department, University of Vermont College of Medicine, Burlington.

Footnotes
Accepted for publication Nov 30, 1979.

Read before the Fourth International Congress on Neuromuscular Disease, Montreal, Sept 18, 1978.

Reprint requests to Pathology Department, University of Vermont College of Medicine, Burlington, VT 05405 (Dr Perl).

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