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Simultaneous Determination of Skeletal Muscle Fiber, Types I, IIA, and IIB by Histochemistry
Gary L. Tunell, MD;
Michael Noel Hart, MD
Arch Neurol. 1977;34(3):171-173.
Abstract
• We describe a simplified technique for the histochemical determination of three fiber types from a single section of skeletal muscle. Preincubation in a solution of formaldehyde, glycine, and calcium followed by routine myofibrillar adenosine triphosphatase (ATPase) incubation clearly differentiates type I, type IIA, and IIB fibers in human, rat, rabbit, and porcine muscle. In addition, glycineformaldehyde-calcium preincubation offers better preservation of cytoarchitecture and standardization of incubation time.
Author Affiliations
From the Department of Pathology, University of Missouri School of Medicine, Columbia, Mo.
Footnotes
Accepted for publication Nov 4, 1976.
Reprint requests to the Department of Pathology, University of Iowa College of Medicine, Iowa City, IA 52242 (Dr Hart).
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