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Passive Transfer by the Intraocular Injection of Sensitized Cells

Shirley H. Wray, MD, PhD; David G. Cogan, MD; Barry G. W. Arnason, MD

Arch Neurol. 1976;33(3):183-185.

Abstract
• Experimental allergic encephalomyelitis is a disease of cell-mediated immunity and can be transferred passively to virgin recipients by lymphoid cells from sensitized donors. The rabbit eye contains myelinated medullary rays that can be visualized ophthalmoscopically. Intraocular injection of autologous lymph node cells from myelin basic protein (BP)-immunized rabbits into the vitreous leads to readily visualized optic neuritis while injection of cells from adjuvant immunized control rabbits does not. Microscopical study confirmed the presence of myelin destruction in recipients of cells from BP-sensitized donors. This eye chamber technique provides a simple model for the study of demyelination in vivo under direct observation.

Author Affiliations
From the Department of Neurology, Massachusetts General Hospital, Boston (Drs Wray and Arnason), and the Clinical Branch, National Eye Institute, Bethesda, Md (Dr Cogan).

Footnotes
Accepted for publication Feb 26, 1975.

Read before the Association for Research in Vision and Ophthalmology, Sarasota, Fla, April 26, 1974.

Reprint requests to 243 Charles St, Boston, MA 02114 (Dr Wray).