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Detection by Immunofluorescence

Eugenia T. Gamboa, MD; Abner Wolf, MD; Melvin D. Yahr, MD; Donald H. Harter, MD; Philip E. Duffy, MD; Herbert Barden, PhD; Konrad C. Hsu, PhD

Arch Neurol. 1974;31(4):228-232.

Abstract
Brains of six patients with postencephalitic parkinsonism were examined for influenza virus antigen by direct immunofluorescence. Tissue sections were exposed to fluorescein-conjugated globulin directed against the NWS, WSN, WS, and PR8 strains of influenza A₀, the ribonucleoprotein of the WSN strain, the swine strain of influenza A, the Japan strain of influenza A₂, the Lee strain of influenza B, measles, and herpes simplex virus.

Intranuclear fluorescent antigen was detected in hypothalamic and midbrain sections prepared from all six cases after staining with antibody to the two neurotropic influenza A₀ strains (NWS and WSN) and to the ribonucleoprotein of the WSN strain.

No fluorescence was observed after staining of similar sections prepared from the brains of five patients with idiopathic Parkinson disease with labeled influenza NSW antiserum.

Author Affiliations
From the departments of neurology (Drs. Gamboa, Yahr, and Harter), microbiology, and pathology (Drs. Wolf, Duffy, Barden, and Hsu), College of Physicians and Surgeons, Columbia University, New York. Dr. Yahr is now with the Department of Neurology, Mount Sinai Medical Center, New York.

Footnotes
Accepted for publication March 7, 1974.

A preliminary report of this study was read before the 98th annual meeting of the American Neurological Association, Montreal, June 12, 1973.

Reprint requests to Department of Neurology, Mount Sinai Medical Center, 100th St and Fifth Ave, New York, NY 10029 (Dr. Yahr).

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