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Michael G. Murphy, MD; James L. O'Leary, MD; David Cornblath

Arch Neurol. 1973;28(2):118-123.

Abstract
Tritiated leucine was injected unilaterally into pontine, inferior olivary, or reticular nuclei of cat brainstem to establish sources of cerebellar mossy and climbing terminals. Allowing 24 to 72 hours for label uptake and active transport by axoplasmic flow to cerebellar cortex, autoradiographs were prepared with the use of parasagittal sections from both sides. Larger volumes (1µI to 5µI) were injected initially to facilitate identification of labeled terminals. Thereafter, smaller volumes were used to obtain more discrete and reliable results. Several types of control eliminated alternative routes of transport. Both inferior olive and pontine nuclei were shown to be sources of contralaterally labeled mossy and climbing terminals, the former somewhat more numerous in pontine injections, the latter significantly so in olivary ones. Reticular sites prinicipally showed climbing terminals. Labeled terminals were also seen homolaterally to the injection, but much less frequently than contralaterally.

Author Affiliations
St. Louis

From the Division of Neurological Surgery and Department of Neurology, Washington University School of Medicine, St. Louis.

Footnotes
Accepted for publication Oct 3, 1972.

Reprint requests to Department of Neurology, Washington University School of Medicine, 660 S Euclid Ave, Box 8111, St. Louis 63110 (Dr. O'Leary).

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