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Distribution of Cyclic Adenosine Monophosphate in Rat Brain
Manuchair S. Ebadi, PhD;
Benjamin Weiss, PhD;
Erminio Costa, MD
Arch Neurol. 1971;24(4):353-357.
Abstract
Cyclic 3',5'-adenosine monophosphate (AMP) was assayed by converting it enzymatically to adenosine triphosphate (ATP) with phosphodiesterase, myokinase, and pyruvate kinase. Adenosine triphosphate was measured in a liquid scintillation spectrometer from the photons emitted when ATP serves as a substrate of the firefly luciferin-luciferase system. In the rat, the highest concentrations of cyclic AMP (35µµmols to 58µµmols\mg protein) were found in the pituitary and pineal glands and in cerebellum; intermediate levels (19µµmols to 25µµmols/gm protein) were found in spinal cord, thalamus, hypothalamus, telencephalon, and olfactory bulb; the lowest concentrations (11µµmols to 15µµmols/ mg protein) were found in corpora quadrigemina, olfactory tubercle, caudate, hippocampus, pons, and medulla. Studies of the subcellular distribution of cyclic AMP showed that virtually all the cyclic AMP was bound to particulate material associated with the nuclear and mitochondrial fractions. of brain homogenates.
Author Affiliations
Washington, DC
From the Laboratory of Preclinical Pharmacology, National Institute of Mental Health, St. Elizabeths Hospital, Washington, DC. Dr. Ebadi is now with the University of Nebraska School of Medicine, Omaha.
Footnotes
Accepted for publication Nov 28, 1970.
Read before the 95th Annual Meeting of the American Neurological Association, Atlantic City, NY, June 17, 1970.
Reprint requests to Laboratory of Preclinical Pharmacology, National Institute of Mental Health, St. Elizabeths Hospital, WAW Bldg, Washington, DC 20032 (Dr. Costa).
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ABSTRACT
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